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Related Concept Videos

  • Biomedical And Clinical Sciences
  • Oncology And Carcinogenesis
  • Predictive And Prognostic Markers
  • Multiple Transcript Cleavage Precedes Polymerase Release In Termination By Rna Polymerase Ii.
  • Biomedical And Clinical Sciences
  • Oncology And Carcinogenesis
  • Predictive And Prognostic Markers
  • Multiple Transcript Cleavage Precedes Polymerase Release In Termination By Rna Polymerase Ii.

    • Related Experiment Videos

    Multiple transcript cleavage precedes polymerase release in termination by RNA polymerase II.

    M J Dye1, N J Proudfoot

    • 1Sir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford OX1 3RE, United Kingdom.

    Cell
    |June 8, 2001

    View abstract on PubMed

    Summary
    This summary is machine-generated.

    RNA polymerase II (pol II) transcription termination involves two distinct events: pretermination cleavage (PTC) mediated by downstream sequences and polymerase disengagement requiring both PTC and a poly(A) site.

    Related Experiment Videos

    Area of Science:

    • Molecular Biology
    • Gene Expression
    • Biochemistry

    Background:

    • Polyadenylation (poly(A)) signals are known to be essential for RNA polymerase II (pol II) transcription termination.
    • The precise mechanisms governing pol II transcription termination remain largely unelucidated.

    Purpose of the Study:

    • To investigate the sequential events involved in RNA polymerase II transcription termination.
    • To elucidate the roles of downstream sequences and poly(A) sites in the termination process.

    Main Methods:

    • Analysis of nascent transcripts from human beta- and epsilon-globin genes.
    • Identification and characterization of pretermination cleavage (PTC) events.

    Main Results:

    • Pol II transcription termination comprises two distinct, temporally separated events.
    • Pretermination cleavage (PTC) is facilitated by sequence tracts downstream of the poly(A) site, causing heterogeneous transcript cleavage.
    • Polymerase disengagement from the DNA template necessitates prior transcription of both a PTC sequence and a functional poly(A) site.

    Conclusions:

    • Transcription termination by RNA polymerase II is a multi-step process.
    • Sequence elements downstream of the poly(A) site play a critical role in initiating termination through pretermination cleavage.
    • Efficient termination requires the coordinated action of both pretermination cleavage and polyadenylation signals.