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Updated: Jul 29, 2026

Analysis of Termination of Transcription Using BrUTP-strand-specific Transcription Run-on (TRO) Approach
Published on: March 12, 2017
Transcriptional termination factors for RNA polymerase II in yeast
1Sir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford OX1 3RE, United Kingdom.
Abstract:
The molecular connections between mRNA 3' end processing and transcriptional termination have been investigated in S. pombe using a genetic screen. By this approach, we have identified a RNAP II termination domain in the well-defined cleavage polyadenylation factor called CstF-64 in metazoans and Rna15p in S. cerevisiae. Furthermore, this C-terminal domain interacts with Res2, previously identified as a component of the G1/S transition-specific transcription factor MBF. Deletion of res2 in both fission and budding yeast results in a defect in 3' end formation. This raises the possibility that RNAP II transcriptional termination may in some situations be integrated with cell cycle events.
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