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Microsatellite stability in human post-mortem tissues.

P Hoff-Olsen1, S Jacobsen, B Mevåg

  • 1Institute of Forensic Medicine, University of Oslo, 0027, Oslo, Norway. per.hoff-olsen@labmed.uio.no

Forensic Science International
|June 8, 2001
PubMed
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Short tandem repeat (STR) DNA profiling is reliable for human identification and forensic cases, even with decomposed samples. This study found STR sequences stable in various tissues, regardless of decomposition, ensuring accurate results.

Area of Science:

  • Forensic Science
  • Genetics
  • Molecular Biology

Background:

  • Human identification and forensic casework often rely on DNA profiling, particularly from decomposed remains.
  • Somatic microsatellite (STR) instability can compromise the accuracy of DNA analyses, leading to potential false inclusions or exclusions.
  • The stability of STR sequences in postmortem tissues and its impact on forensic analysis require further investigation.

Purpose of the Study:

  • To systematically assess the somatic and postmortem stability of short tandem repeat (STR) sequences in human tissues.
  • To determine the reliability of PCR-based STR analyses in forensic casework involving decomposed biological samples.

Main Methods:

  • DNA was extracted from blood and six tissue samples from 26 deceased individuals without decomposition and 25 with decomposition.

Related Experiment Videos

  • Seven STR loci (tetranucleotides: HUMVWA31/A, HUMTH01, HUMF13A1, HUMFES/FPS; hyperpolymorphic: HUMAPOAI1, D11S554, HUMACTBP2) were analyzed.
  • DNA profiles were generated using denaturing gel electrophoresis on an ABD Prism 377 gene sequencer, with bone DNA profiles serving as standards.

    • Main Results:

    • In cases without decomposition, STR sequences demonstrated stability, showing no evidence of somatic mutations across different tissues.
    • Cases with varying degrees of postmortem decomposition exhibited microsatellite stability.
    • No somatic mutations or postmortem changes leading to inter-organ non-matches were detected in decomposed samples.

    Conclusions:

    • PCR-based STR analyses are suitable for human identification and forensic casework, even when dealing with heavily decomposed tissues.
    • The stability of STR sequences in both fresh and decomposed human tissues supports their reliability in forensic applications.
    • The findings confirm that STR profiling can be confidently used for identifying individuals and in criminal investigations involving degraded DNA samples.