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Related Experiment Videos

A multiprotein complex that interacts with RNA polymerase II elongator.

Y Li1, Y Takagi, Y Jiang

  • 1Department of Structural Biology, Stanford University School of Medicine, Stanford, California 94305-5400, USA. yli@tularik.com

The Journal of Biological Chemistry
|June 8, 2001
PubMed
Summary
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Researchers identified a yeast Hap complex interacting with RNA polymerase II Elongator. Gene deletions revealed Hap complex involvement in regulating Elongator activity and cellular responses.

Area of Science:

  • Molecular Biology
  • Yeast Genetics
  • Protein Interactions

Background:

  • The Elongator complex is crucial for transcription and other cellular processes.
  • The Hap complex is known to regulate gene expression in yeast.
  • Interactions between transcription factors and RNA polymerase II machinery are vital for gene regulation.

Purpose of the Study:

  • To investigate the interaction between the Hap complex and RNA polymerase II Elongator in yeast.
  • To elucidate the functional significance of this interaction.
  • To identify the specific Hap subunits involved in the interaction and their roles.

Main Methods:

  • Isolation of a three-subunit Hap complex from yeast.
  • Genetic analysis using gene deletions for Hap subunits (HAP1, HAP3).

Related Experiment Videos

  • Phenotypic analysis of deletion mutants, including pGKL killer sensitivity and Elongator function assays.
  • Biochemical assays to study the interaction between the Hap complex and Elongator.

    • Main Results:

    • A three-subunit Hap complex that interacts with RNA polymerase II Elongator was successfully isolated.
    • Deletion of HAP1 or HAP3 genes resulted in pGKL killer-insensitive phenotypes.
    • These deletions also led to a weak Elongator phenotype, indicating functional involvement.
    • The Hap complex preferentially interacts with free Elongator over RNA polymerase II-associated Elongator.

    Conclusions:

    • The Hap complex plays a regulatory role in Elongator activity.
    • Specific Hap subunits (HAP1 and HAP3) are essential for proper Elongator function and cellular responses.
    • The interaction dynamics suggest a mechanism for modulating Elongator function in response to cellular signals.