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Updated: Oct 7, 2026

A Micropatterning Assay for Measuring Cell Chirality
Published on: March 11, 2022
Simultaneous microbatch screening of enantiorecognition on solid chiral selectors using selected mixtures of
C Roussel1, B Bonnet, I De Riggi
1Laboratoire Stéréochimie Dynamique et Chiralité, ENSSPICAM, UMR CNRS 6516, Université, 13397 Marseille Cedex 20, France. roussel@u3pic105.u-3mrs.fr
Abstract:
An improved methodology for microbatch screening in the liquid-solid enantioselective adsorption of racemates by solid chiral selector is presented and illustrated by the evaluation of a series of six cellulose tris(alpha-phenylpropionate) presenting a configurational diversity and cellulose tris (4-methylbenzoate). Analyses were performed on 5 mg scale of chiral selector and 150 microL of supernatant containing a mixture of three test-racemates. Fifteen test-racemates were chosen according to their molecular diversity using a hierarchical clustering approach for seven selected three-dimensional molecular properties. The 15 racemates were sorted in five test mixtures composed of three racemates each according to the following constraints: each triplet of test-racemates can be analyzed without peak overlap on a commercially available chiral stationary phase (CSP). The designed five groups of three racemates may be used for the evaluation of other chiral selectors in view of the preparation of CSPs. This methodology improves the throughput of the evaluation and could be automated.
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