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Synthesis and properties of oligonucleotides containing 8-bromo-2'-deoxyguanosine
C Fàbrega1, M J Macías, R Eritja
1European Molecular Biology Laboratory, Heidelberg, Germany.
Nucleosides, Nucleotides & Nucleic Acids
|June 8, 2001
Abstract:
The preparation of oligonucleotides containing 8-bromo-2'-deoxyguanosine is described. Substitution of G by 8-bromoguanine on an alternating CG decamer stabilizes the Z-form in such a way that the B-form was not observed. Melting temperatures showed that duplexes in which 8-bromo-2'-deoxyguanosine paired with natural bases were much less stable.