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Preparation of recombinant coat protein of Prunus necrotic ringspot virus
K Petrzik1, I Mráz, D Kubelková
1Department of Plant Virology, Institute of Plant Molecular Biology, Academy of Sciences of the Czech Republic, Ceské Budejovice.
Acta Virologica
|June 8, 2001
Abstract:
The coat protein (CP) gene of Prunus necrotic ringspot virus (PNRSV) was cloned into pET 16b vector and expressed in Escherichia coli. CP-enriched fractions were prepared from whole cell lysate by differential centrifugation. The fraction sedimenting at 20,000 x g for 30 mins was used for preparation of a rabbit antiserum to CP. This antiserum had a titer of 1:2048 and reacted in a double-antibody sandwich ELISA (DAS-ELISA).
