Related Experiment Videos
[Antimicrobial resistance of coliform isolates from expressed human milk]
F R Novak1, J A Almeida, M D Asensi
1Banco de Leite Humano, Instituto Fernandes Figueira, Fundação Oswaldo Cruz, Rio de Janeiro, RJ, 22250-020, Brasil. novak@iff.fiocruz.br
Abstract:
The dispersion of potentially pathogenic, antibiotic-resistant microorganisms via expressed human milk can be considered a risk factor. The aim of this study was to contribute to a better understanding of coliform isolates from expressed human milk and their antimicrobial resistance profiles. The sampling scheme followed a totally randomized design, using 837 samples of expressed human milk. Of these, 71 (8.48%) were identified as contaminated with total coliforms, although in none of the samples did the population exceed 1.0x10(3) MPN/ml. Most of the microorganisms isolated (91.6%) belonged to only two species, Enterobacter cloacae and Klebsiella pneumoniae, which when subjected to antibiograms, revealed that several strains showed prior resistance to some of the antimicrobials tested. Coliforms may grow in expressed human milk if it is improperly stored, depleting protection factors and reducing the milk's nutritional value.
Insights
Antibiotic-resistant coliforms, primarily Enterobacter cloacae and Klebsiella pneumoniae, were found in expressed human milk. Improper storage can lead to bacterial growth, reducing milk
Area of Science:
- Microbiology
- Public Health
- Food Safety
Background:
- Expressed human milk can harbor pathogenic microorganisms.
- Antibiotic-resistant bacteria pose a significant public health risk.
- Understanding microbial contamination in human milk is crucial for infant health.
Purpose of the Study:
- To investigate coliform contamination in expressed human milk.
- To determine the antimicrobial resistance profiles of isolated coliforms.
- To assess the risk associated with microbial presence in human milk.
Main Methods:
- Analysis of 837 expressed human milk samples using a randomized design.
- Identification of coliform isolates and their species.
- Antibiogram testing to evaluate antimicrobial resistance patterns.
Main Results:
- 71 (8.48%) milk samples showed coliform contamination, with populations below 1.0x10(3) MPN/ml.
- Enterobacter cloacae and Klebsiella pneumoniae were the predominant species (91.6%).
- Multiple strains exhibited pre-existing resistance to tested antimicrobials.
Conclusions:
- Coliforms, particularly E. cloacae and K. pneumoniae, can contaminate expressed human milk.
- Improper milk storage facilitates coliform proliferation, compromising milk quality and nutritional value.
- Findings highlight the importance of safe handling and storage of expressed human milk to prevent microbial contamination and resistance spread.