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Identification and analysis of caspase substrates: proteolytic cleavage of poly(ADP-ribose)polymerase and DNA

C Boucher1, S Gobeil, K Samejima

  • 1Health and Environment Unit, Laval University Medical Research Center, CHUQ and Faculty of Medicine, Laval University, Quebec, Canada G1V 4G2.

Methods in Cell Biology
|June 9, 2001
PubMed
Abstract

No abstract available in PubMed .

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Caspases

Caspase, a family of cysteine proteases, serve as effectors in apoptosis. The ced3 gene in C.elegans was first identified to be involved in apoptosis. This gene encodes the ced-3 caspase that is similar to the interleukin-1-beta converting enzyme or ICE in mammals. In addition to apoptosis, caspases also function in the inflammatory response. Inflammatory caspases are essential in activating pro-inflammatory cytokines that recruit immune cells and block the replication of pathogens inside cells.
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Since the discovery of the two BER pathways, there has been a debate about how a cell chooses one pathway over the other and the factors determining this selection. Numerous in vitro experiments have pointed out multiple determinants for the sub-pathway selection. These are:

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