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Translocation of S100A1(1) calcium binding protein during heart surgery
W Brett1, A Mandinova, A Remppis
1Division of Cardio-Thoracic Surgery, University of Basel, Kantonsspital, CH-4031 Basel, Switzerland. wbrett@uhbs.ch
Insights
Heart-specific S100A1 protein is involved in myocardial damage during open heart surgery. This calcium-binding protein may act as an intracellular link in ischemia-reperfusion injury, impacting heart muscle cells.
Area of Science:
- Cardiology
- Molecular Biology
- Biochemistry
Background:
- Myocardial ischemia during cardiopulmonary bypass leads to cardiomyocyte damage via calcium overload.
- Heart-specific calcium-binding proteins are implicated in ischemia-reperfusion injury.
- S100A1 is a key calcium-binding protein in myocytes, influencing various calcium-mediated functions.
Purpose of the Study:
- To investigate the localization and translocation of S100A1 in the human heart.
- To determine the role of S100A1 in myocardial ischemia-reperfusion injury during open heart surgery.
Main Methods:
- Study involved human heart tissue samples.
- Analysis of S100A1 localization under normal and ischemia-reperfusion conditions.
Main Results:
- S100A1 exhibits specific localization patterns in human heart myocytes.
- Data suggest S100A1 is directly involved in perioperative myocardial damage.
- S100A1 translocation may occur during ischemia and reperfusion.
Conclusions:
- S100A1 plays a significant role in the transient myocardial damage associated with open heart surgery.
- S100A1 may serve as a critical intracellular mediator in cardiac ischemia-reperfusion injury.
- Understanding S100A1's function could lead to novel therapeutic strategies for heart protection.
Abstract:
Myocardial ischemia during cardiopulmonary bypass terminated by reperfusion generally leads to different degrees of damage of the cardiomyocytes induced by transient cytosolic Ca(2+) overload. Recently, much attention has been paid to the role of heart-specific Ca(2+)-binding proteins in the pathogenesis of myocardial ischemia-reperfusion injury. S100A1 is a heart-specific EF-hand Ca(2+)-binding protein that is directly involved in a variety of Ca(2+)-mediated functions in myocytes. The aim of our study was to investigate the localization and translocation of S100A1 in the human heart under normal (baseline) conditions and after prolonged ischemia and reperfusion of the myocardium. Our data suggest that S100A1 is directly involved in the transient perioperative myocardial damage caused by ischemia during open heart surgery in humans. Given its role in the contractile function of muscle cells, this S100 protein could be an important "intracellular link" in ischemia-reperfusion injury of the heart.