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Updated: Oct 7, 2026
qPCR Is a Sensitive and Rapid Method for Detection of Cytomegaloviral DNA in Formalin-fixed, Paraffin-embedded Biopsy Tissue
Published on: July 9, 2014
Use of nested polymerase chain reaction (PCR) for detection of retroviruses from formalin-fixed, paraffin-embedded
J. Stiles1, D. Bienzle, J.A. Render
1Department of Small Animal Medicine, University of Georgia, College of Veterinary Medicine, Athens, Georgia 30602, USA; Department of Pathology, University of Georgia, College of Veterinary Medicine, Athens, Georgia 30602, USA; Animal Health Diagnostic Laboratory and the Department of Pathology, Michigan State University, College of Veterinary Medicine, East Lansing MI 48824, USA; Department of Surgical and Radiological Sciences, University of California, School of Veterinary Medicine, Davis, CA 95616, USA.
Abstract:
Thirty-six formalin-fixed, paraffin-embedded enucleated globes from cats with a diagnosis of diffuse anterior uveal melanoma were obtained. Sections of tumor were excised, deparaffinized, and subjected to nested polymerase chain reaction (PCR) to identify proviral DNA sequences from the feline leukemia virus (FeLV)-feline sarcoma virus (FeSV; 36 eyes), and the feline immunodeficiency virus (FIV; 18 eyes). All samples tested were negative for FIV DNA. Three samples were positive for FeLV-FeSV DNA. This is the first reported evidence of a possible link between naturally occurring feline anterior uveal melanoma and the presence of FeLV-FeSV DNA.
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