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Broth Microdilution In Vitro Screening: An Easy and Fast Method to Detect New Antifungal Compounds
Published on: February 14, 2018
Antifungal polysulphides from Petiveria alliacea L
P J Benevides1, M C Young, A M Giesbrecht
1Instituto de Química, Universidade de São Paulo, C.P. 26077, CEP 05599-970, SP, Brazil.
Abstract:
Bioactivity-directed fractionation of the CH(2)Cl(2)/MeOH (2:1, v/v) extract of the roots of Petiveria alliacea, using mutant yeast strains of Saccharomyces cerevisiae and fungi Cladosporium cladosporioides and C. sphaerospermum led to the isolation of dipropyl disulphide (1), dibenzyl sulphide (2), dibenzyl disulphide (3), dibenzyl trisulphide (4), dibenzyl tetrasulphide (5), benzylhydroxymethyl sulphide (6) and di(benzyltrithio) methane (7). Of these, 5-7 are new compounds and this is the first report of the natural occurrence of 2 and 3.
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