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Related Experiment Videos

Efficient polycyclic aromatic hydrocarbons dihydroxylation in direct micellar systems.

D Randazzo1, D Berti, F Briganti

  • 1Laboratorio di Chimica Bioinorganica, Dipartimento di Chimica, Università di Firenze, Via Della Lastruccia 5, 50019 Florence, Italy.

Biotechnology and Bioengineering
|June 16, 2001
PubMed
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This study optimizes polycyclic aromatic hydrocarbon (PAH) bioconversion using engineered E. coli in micellar systems. Direct microemulsions enhance efficiency and yields for naphthalene, anthracene, and phenanthrene degradation.

Area of Science:

  • Biotechnology and Biocatalysis
  • Environmental Microbiology
  • Chemical Engineering

Background:

  • Polycyclic Aromatic Hydrocarbons (PAHs) are persistent environmental pollutants.
  • Whole-cell bioconversion offers a sustainable method for PAH remediation.
  • Escherichia coli JM109 (pPS1778) engineered with Pseudomonas fluorescens N3 genes shows potential for PAH degradation.

Purpose of the Study:

  • To optimize whole-cell bioconversion of anthracene, phenanthrene, and naphthalene into enantiomerically pure cis-dihydroxydihydro derivatives.
  • To investigate the efficacy of direct microemulsion systems for enhancing bioconversion efficiency and product yields.
  • To understand and mitigate product inhibition in PAH bioconversion.

Main Methods:

  • Utilized a recombinant Escherichia coli strain expressing naphthalene dioxygenase and regulatory genes.

Related Experiment Videos

  • Employed direct microemulsion systems with nonionic surfactant (Triton X-100) and selected oils to solubilize PAHs.
  • Monitored microemulsion phase behavior using light scattering experiments.
  • Quantified bioconversion efficiency and product yields for naphthalene, anthracene, and phenanthrene.

    • Main Results:

    • Direct microemulsions effectively solubilized high concentrations of PAHs, enhancing bioconversion efficiency.
    • Achieved 100% yield for naphthalene, >60% for phenanthrene, and >30% for anthracene.
    • Identified reversible product inhibition for anthracene and phenanthrene dihydroxylated derivatives.
    • Demonstrated that product removal can restore bioconversion rates.

    Conclusions:

    • Direct microemulsion systems are effective for optimizing whole-cell bioconversion of PAHs by engineered E. coli.
    • The developed system significantly improves substrate solubilization and bioconversion yields.
    • Strategies for product separation are necessary to overcome reversible inhibition and achieve complete PAH conversion.