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Nonradioactive determination of Ras-GTP levels using activated ras interaction assay

S J Taylor1, R J Resnick, D Shalloway

  • 1Department of Molecular and Cell Biology, University of California, Berkeley, California 94720, USA.

Methods in Enzymology
|June 13, 2001
PubMed
Abstract

No abstract available in PubMed .

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The Ras Gene02:38

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The Ras-gene-encoded proteins are regulators of signaling pathways controlling cell proliferation, differentiation, or cell survival. The Ras-gene family in humans constitutes three primary members—the HRas, NRas, and KRas. These genes code for four functionally distinct yet closely related proteins—the HRas, NRas, KRas4A, and KRas4B. The involvement of mutant Ras genes in human cancer was first discovered in 1982 and is among the most common causes of human tumorigenesis.
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Ras and Rho are small monomeric GTPases that act downstream of receptor tyrosine kinase (RTK) and regulate various cellular processes. These GTPases switch between active and inactive states by binding to guanine nucleotides.
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