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The onset of collagen synthesis in sea urchin embryos
R Golob1, C J Chetsanga, P Doty
1Department of Biochemistry and Molecular Biology, Harvard University, Cambridge, Mass. 02138, USA.
Biochimica Et Biophysica Acta
|April 27, 1974
Abstract:
In Arbacia punctulata and Strongylocentrotus purpuratus, two species of sea urchins, collagen synthesis begins during gastrulation and increases many-fold before reaching a plateau in the late pluteus stage. A collagen extraction method involving treatment with 0.1 M NaOH and hot 10% trichloroacetic acid provided the basis for a sensitive assay of collagen synthesis.