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qPCR Is a Sensitive and Rapid Method for Detection of Cytomegaloviral DNA in Formalin-fixed, Paraffin-embedded Biopsy Tissue
Published on: July 9, 2014
Evaluation of formalin-fixed paraffin-embedded tissues from vaccine site-associated sarcomas of cats for polyomavirus
B A Kidney1, D M Haines, J A Ellis
1Department of Veterinary Pathology, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, Canada.
Objective:
To determine whether vaccine site-associated sarcomas (VSS) from cats contain polyomavirus antigen or DNA.
Sample Population:
50 formalin-fixed paraffin-embedded tissue blocks of VSS from cats.
Procedure:
Sections from each tissue block were evaluated for polyomavirus antigen by use of an avidin-biotin-complex immunohistochemical staining method, using rabbit anti-murine polyomavirus polyclonal antiserum as the primary antibody. The DNA was extracted from sections of each tissue block, and a polymerase chain reaction assay was performed, using primers designed to amplify regions of the bovine polyomavirus genome and consensus polyomavirus primers designed to detect unknown polyomaviruses.
Results:
Polyomavirus antigen and DNA were not detected in any of the VSS.
Conclusions And Clinical Relevance:
Results suggest that polyomaviruses likely do not have any direct involvement in the pathogenesis of VSS in cats.
