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Development of a gene inactivation system for Bacteroides forsythus: construction and characterization of a BspA
K Honma1, H K Kuramitsu, R J Genco
1Department of Oral Biology, School of Dental Medicine, State University of New York at Buffalo, Buffalo, New York 14214, USA.
Abstract:
Bacteroides forsythus is a gram-negative anaerobic bacterium associated with periodontitis. The bspA gene encoding a cell surface associated leucine-rich repeat protein (BspA) involved in adhesion to fibronectin and fibrinogen was recently cloned from this bacterium in our laboratory. We now describe the construction of a BspA-defective mutant of B. forsythus. This is the first report describing the generation of a specific gene knockout mutant of B. forsythus, and this procedure should be useful in establishing the identity of virulence-associated factors in these organisms.
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