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Updated: Jul 27, 2026
Broth Microdilution In Vitro Screening: An Easy and Fast Method to Detect New Antifungal Compounds
Published on: February 14, 2018
In vitro studies of the efficacy of antimicrobials against fungi
T F Meiller1, J I Kelley, M A Jabra-Rizk
1Department of Oral Medicine and Diagnostic Sciences, Unversity of Maryland Dental School, Balitmore, Md 21201, USA. TFM001@dental.umaryland.edu
Objective:
The purpose of this study was to compare the efficacy of Listerine Antiseptic, Tartar Control Listerine Antiseptic, and Peridex mouthrinses and a 0.2% chlorhexidine digluconate solution against known pathogenic fungi.
Study Design:
Standardized methods were used to compare the antimicrobial efficacy of the above agents versus representative fungal species. Minimum inhibitory concentration-minimum fungicidal concentrations in macrobroth dilutions, suspension kill-time, and effectiveness against an artificial biofilm-attached population were studied.
Results:
All antimicrobials tested were effective against the fungal species under investigation at the concentration available commercially. Listerine Antiseptic showed a greater efficacy against attached artificial biofilm populations than the other antimicrobials tested.
Conclusions:
Listerine Antiseptic, Tartar Control Listerine Antiseptic, and Peridex mouthrinses show promise as a means to control the pathogenic fungal species under investigation and may have applications to reduce oral colonization.
Insights
Listerine Antiseptic and other mouthrinses effectively combat pathogenic fungi. Listerine Antiseptic demonstrated superior performance against fungal biofilms, suggesting potential for reducing oral fungal colonization.
Area of Science:
- Oral microbiology
- Antimicrobial efficacy testing
- Mycology
Background:
- Pathogenic fungi can cause oral infections.
- Antiseptic mouthrinses are commonly used for oral hygiene.
- Controlling fungal growth in the oral cavity is important for preventing infections.
Purpose of the Study:
- To compare the antifungal efficacy of Listerine Antiseptic, Tartar Control Listerine Antiseptic, and Peridex mouthrinses against pathogenic fungi.
- To evaluate a 0.2% chlorhexidine digluconate solution as a control.
Main Methods:
- Standardized antimicrobial efficacy testing methods were employed.
- Minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) were determined using macrobroth dilutions.
- Suspension kill-time assays and effectiveness against artificial biofilm-attached fungal populations were assessed.
Main Results:
- All tested mouthrinses and the chlorhexidine solution exhibited efficacy against the target fungal species at commercial concentrations.
- Listerine Antiseptic demonstrated superior effectiveness in eliminating artificial biofilm-attached fungal populations compared to the other agents.
Conclusions:
- Listerine Antiseptic, Tartar Control Listerine Antiseptic, and Peridex mouthrinses show potential for controlling pathogenic oral fungi.
- These mouthrinses may be beneficial in reducing oral fungal colonization and preventing associated infections.
