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In vivo nuclear delivery of oligonucleotides via hybridizing bifunctional peptides
L J Brandén1, B Christensson, C I Smith
1Department of Biosciences, Karolinska Institutet, NOVUM, and Department of Immunology, Microbiology, Pathology and Infectious Diseases, Huddinge University Hospital, Huddinge, Sweden.
Abstract:
Linking proteins directly to nucleic acids has been a complex task. By hybridizing a bifunctional peptide nucleic acid (PNA) consisting of a nucleic acid binding moiety and a nuclear localization signal (NLS) we have previously demonstrated that it is possible to link protein functions directly to nucleic acids containing a PNA target site. By hybridizing fluorescently labeled oligonucleotides to PNA-NLS molecules and subsequently transfecting different organs in vivo we demonstrate an active nuclear translocation of the PNA-NLS/oligonucleotide complex in different mouse organs.