[Comparison of denaturing high performance liquid chromatography with direct sequencing in the detection of single
1Chinese Human Genome Center at Shanghai, Shanghai 201203 P. R. China. huangwei@chgc.sh.cn
Objective:
Investigate the sensitivity and accuracy of the denaturing high performance liquid chromatography(DHPLC) technique for the detection of single nucleotide polymorphism(SNP).
Methods:
Forty-one samples were detected by both DHPLC and direct sequencing.
Results:
The comparison demonstrated that DHPLC detected all heterozygous sequences found by direct sequencing. No false-positive signals were seen in the cases of homozygous sequences. Furthermore, no false-negative results were ever obtained with heterozygous mutations or polymorphisms, or both.
Conclusion:
DHPLC is a potent method for SNP identification especially SNP typing in large scale screening.
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