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Peripheral circulating insulin-like growth factor-I and -II in cattle
J A Nikolić1, O Nedić, H Samanc
1INEP-Institute for the Application of Nuclear Energy, Banatska 31B, 11080 Zemun, Yugoslavia. anikolic@inep.co.yu
Acta Veterinaria Hungarica
|June 14, 2001
Summary
Investigating insulin-like growth factors (IGF-I and IGF-II) in cattle revealed distinct binding protein profiles. Total IGF concentrations varied significantly between male and female cattle, with a reciprocal relationship most evident in periparturient cows.
Area of Science:
- Veterinary Endocrinology
- Animal Physiology
- Biochemistry
Background:
- Insulin-like growth factors (IGF-I and IGF-II) play crucial roles in animal growth and metabolism.
- Understanding the interrelationships and variations of IGF-I and IGF-II in cattle is essential for livestock management and health.
- Circulating IGF binding proteins (IGFBPs) influence IGF bioavailability and activity.
Purpose of the Study:
- To investigate the interrelationships between circulating concentrations of IGF-I and IGF-II in healthy cattle.
- To analyze variations in IGF-I and IGF-II levels based on sex, age, breed, and physiological status.
- To examine the influence of IGF binding protein profiles on IGF concentrations.
Main Methods:
- Analysis of 235 blood samples from 145 healthy beef and dairy cattle (calves, bulls, cows) of various breeds and ages.
- Utilized Western ligand blot autoradiography to determine IGF binding protein profiles.
- Employed validated IGF radioimmunoassays, including validation by molecular sieve chromatography.
Main Results:
- Distinct IGF binding protein profiles were observed between different cattle categories.
- In female cattle, mean IGF-I ranged from 5.1 to 20.5 nmol/l, and IGF-II from 14.7 to 30.0 nmol/l.
- In male cattle, mean IGF-I ranged from 8.2 to 67.4 nmol/l, and IGF-II from 11.9 to 22.9 nmol/l.
- Total molar IGF concentrations were more stable in females (24.7–35.1 nmol/l) than in males (27.3–81.8 nmol/l).
- A reciprocal relationship between serum IGF-I and IGF-II was most apparent in periparturient cows.
Conclusions:
- Circulating IGF-I and IGF-II concentrations exhibit significant variability in cattle, influenced by sex, age, and breed.
- IGF binding protein profiles differ across cattle categories, potentially modulating IGF actions.
- The observed reciprocal relationship between IGF-I and IGF-II, particularly in periparturient cows, warrants further investigation into their dynamic interplay during physiological transitions.