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Cellular immune responses in mice immunized with Anisakis simplex larval antigens
M J Perteguer1, M Rodero, J M Flores
1Departamento de Parasitología, Facultad de Farmacia, Universidad Complutense, Madrid, Spain.
Parasitology Research
|June 14, 2001
Summary
Cellular immune responses to Anisakis simplex infection were studied in mice. Anisakis simplex crude extract induced T cell proliferation and altered immune cell populations, with gastric and intestinal lesions observed in infected mice.
Area of Science:
- Immunology
- Parasitology
Background:
- Anisakis simplex is a parasitic nematode causing anisakiasis.
- Understanding the host immune response is crucial for managing parasitic infections.
Purpose of the Study:
- To investigate cellular immune responses to Anisakis simplex L3 antigens in BALB/c mice.
- To analyze the effects of Anisakis simplex crude extract on immune cell populations and lymph node responses.
Main Methods:
- Mice were immunized subcutaneously with Anisakis simplex crude extract (CE).
- Popliteal lymph node (PLN) cellularity and T cell proliferation were assessed.
- Spleen cells were analyzed by flow cytometry for T cell subsets (CD4+, CD8+, TCRalphabeta+).
- Histological analysis of gastric and intestinal tissues was performed after oral infection.
Main Results:
- Anisakis simplex CE injection increased PLN size and weight.
- In vitro proliferation assays showed responses in PLN cells and T cells.
- Immunization led to increased CD4+ and TCRalphabeta+ T cells.
- Infection altered B cell and T cell populations, with notable changes in CD4+ and CD8+ T cells depending on immunization status.
- Oral infection resulted in gastric and intestinal lesions.
Conclusions:
- Anisakis simplex antigens elicit significant cellular immune responses in mice.
- The parasite modulates T cell populations, with varying effects based on prior exposure.
- Anisakis simplex infection causes distinct pathological changes in the gastrointestinal tract.