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Screening phage-displayed combinatorial peptide libraries
B K Kay1, J Kasanov, M Yamabhai
1Department of Pharmacology, University of Wisconsin-Madison, 1300 University Avenue, Madison, Wisconsin 53706-1532, USA. bkkay@facstaff.wisc.edu
Abstract:
Among the many techniques available to investigators interested in mapping protein-protein interactions is phage display. With a modest amount of effort, time, and cost, one can select peptide ligands to a wide array of targets from phage-display combinatorial peptide libraries. In this article, protocols and examples are provided to guide scientists who wish to identify peptide ligands to their favorite proteins.
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