Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Screening phage-displayed combinatorial peptide libraries.

B K Kay1, J Kasanov, M Yamabhai

  • 1Department of Pharmacology, University of Wisconsin-Madison, 1300 University Avenue, Madison, Wisconsin 53706-1532, USA. bkkay@facstaff.wisc.edu

Methods (San Diego, Calif.)
|June 14, 2001
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Application of Recombinant Human scFv Antibody as a Powerful Tool to Monitor Nitrogen Fixing Biofertilizer in Rice and Legume.

Microbiology spectrum·2021
Same author

pMINERVA: A donor-acceptor system for the in vivo recombineering of scFv into IgG molecules.

Journal of immunological methods·2016
Same author

Generating a panel of highly specific antibodies to 20 human SH2 domains by phage display.

Protein engineering, design & selection : PEDS·2010
Same author

Mapping protein-protein interactions with combinatorial peptides.

Comparative and functional genomics·2008
Same author

Endocytic protein intersectin-l regulates actin assembly via Cdc42 and N-WASP.

Nature cell biology·2001
Same author

Identification of enzyme inhibitors from phage-displayed combinatorial peptide libraries.

Combinatorial chemistry & high throughput screening·2001
Same journal

An accessible, absorbance-based plate reader assay to assess cumulative exposure of blood plasma & serum to thawed conditions.

Methods (San Diego, Calif.)·2026
Same journal

EC-isHCR: A rapid method for in situ hybridization chain reaction in diverse animal samples.

Methods (San Diego, Calif.)·2026
Same journal

Single-Molecule methods to investigate mechanisms of transcription by RNA polymerase of Mycobacterium tuberculosis.

Methods (San Diego, Calif.)·2026
Same journal

Detection and sequencing of Usutu virus during mosquito surveillance: Use of multiple assays and techniques for identification at low levels.

Methods (San Diego, Calif.)·2026
Same journal

Experimental validation of an AI-driven digital healthcare platform for oral health behavior and plaque assessment among vietnamese children.

Methods (San Diego, Calif.)·2026
Same journal

Zeta potential: An efficient and cost-effective alternative for investigating cell-surface interactions.

Methods (San Diego, Calif.)·2026
See all related articles

Phage display is a cost-effective method for identifying peptide ligands to target proteins. This technique enables researchers to map protein-protein interactions efficiently using combinatorial peptide libraries.

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Proteomics

Background:

  • Protein-protein interactions are crucial for cellular functions.
  • Mapping these interactions is essential for understanding biological processes.
  • Various techniques exist, but some are costly or time-consuming.

Purpose of the Study:

  • To provide a guide for scientists using phage display.
  • To demonstrate the selection of peptide ligands to target proteins.
  • To facilitate the mapping of protein-protein interactions.

Main Methods:

  • Utilizing phage-display combinatorial peptide libraries.
  • Selecting peptide ligands against specific protein targets.
  • Detailed protocols and practical examples are included.

Related Experiment Videos

Main Results:

  • Successful identification of peptide ligands is achievable.
  • Phage display offers a high-throughput screening approach.
  • The method is adaptable for diverse protein targets.

Conclusions:

  • Phage display is an accessible and efficient technique.
  • It enables cost-effective mapping of protein-protein interactions.
  • Researchers can readily apply these protocols to their studies.