Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Microbial dehalogenation.

D B Janssen1, J E Oppentocht, G J Poelarends

  • 1Department of Biochemistry, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Nijenborgh 4, NL-9747 AG, Groningen, The Netherlands. d.b.janssen@chem.rug.nl

Current Opinion in Biotechnology
|June 19, 2001
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Mutation of Residue βF71 of Escherichia coli Penicillin Acylase Results in Enhanced Enantioselectivity and Improved Catalytic Properties.

Acta naturae·2012
Same author

The chemical versatility of the beta-alpha-beta fold: catalytic promiscuity and divergent evolution in the tautomerase superfamily.

Cellular and molecular life sciences : CMLS·2008
Same author

Enantioselective formation and ring-opening of epoxides catalysed by halohydrin dehalogenases.

Biochemical Society transactions·2006
Same author

Effect of Chlorinated Ethene Conversion on Viability and Activity of Methylosinus trichosporium OB3b.

Applied and environmental microbiology·2006
Same author

Effect of trichloroethylene on the competitive behavior of toluene-degrading bacteria.

Applied and environmental microbiology·2005
Same author

Multidrug transporters in lactic acid bacteria.

Mini reviews in medicinal chemistry·2005

Novel dehalogenases from bacteria break down halogenated compounds. Studies reveal their molecular mechanisms and evolutionary links to other enzymes, offering insights into biodegradation pathways.

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Enzymology

Background:

  • Halogenated compounds are environmental pollutants.
  • Bacteria possess dehalogenase enzymes to metabolize these compounds.
  • Understanding dehalogenase mechanisms is crucial for bioremediation.

Purpose of the Study:

  • To investigate novel bacterial dehalogenases.
  • To elucidate the molecular mechanisms of dehalogenase activity.
  • To explore the evolutionary relationships of dehalogenases.

Main Methods:

  • X-ray crystallography for structural analysis.
  • Sequence analysis to identify homologous enzymes.
  • Genetic and biochemical studies to characterize enzyme function.

Related Experiment Videos

Main Results:

  • Novel dehalogenases identified in bacteria utilizing halogenated substrates.
  • X-ray and sequence analyses provided insights into hydrolytic dehalogenase mechanisms.
  • Reductive dehalogenases characterized as extracellular, corrinoid-containing iron-sulfur proteins.
  • Sequence and mutagenesis studies revealed homology to enzymes acting on non-halogenated substrates.

Conclusions:

  • Dehalogenases exhibit diverse mechanisms and structures.
  • Evolutionary links suggest a broader role for these enzymes in metabolism.
  • Findings contribute to understanding biodegradation of halogenated pollutants.