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Intersexual partial diploids of phycomyces.

B J Mehta¹, E Cerdá-Olmedo

¹Departamento de Genética, Universidad de Sevilla, 41080 Seville, Spain.

|June 19, 2001

Summary

Anomalous Phycomyces blakesleeanus segregants exhibited sexual stimulation without a partner, revealing diploidy and altering the sexual cycle. Some strains produced high beta-carotene yields, suggesting practical applications in carotene production.

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Area of Science:

Mycology
Fungal Genetics
Biochemistry

Background:

Sexual reproduction in Mucorales fungi triggers hyphal changes and increases carotene content.
Anomalous segregants in Phycomyces blakesleeanus display sexual stimulation characteristics without mating partners.

Purpose of the Study:

To analyze the genetic makeup of anomalous segregants in Phycomyces blakesleeanus.
To investigate the implications of these segregants on the fungal sexual cycle and carotene production.

Main Methods:

Genetic analysis of segregants from crosses involving carF mutations and other markers.
Characterization of diploidy and partial diploidy in the segregants.
Monitoring of nuclear composition changes through selection of individual nuclei.

Main Results:

Identified diploid and partial diploid segregants heterozygous for sex markers, a novel finding in Zygomycetes.
Observed random chromosome losses leading to heterokaryosis and sectoring in coenocytic mycelia.
Demonstrated that some intersexual strains with carF mutations accumulate significant amounts of beta-carotene (25 mg/g dry mass).

Conclusions:

The findings necessitate a reinterpretation of the sexual cycle in Phycomyces.
The stability and high beta-carotene yield of certain intersexual strains present potential for industrial carotene production.

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