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Effect of diabetes and insulin treatment on nitric oxide synthase content in rat corpus cavernosum

Z S Xu¹, Q Fu, S T Zhao

¹Department of Urology, Qilu Hospital, Shandong University, Jinan, China. xianzhouj@163.net

Asian Journal of Andrology

|June 19, 2001

Abstract

Aim:

To study the effect of diabetes mellitus and insulin treatment on rat penile nitric oxide synthase content.

Methods:

Male Wistar rats were divided at random into two groups: the Control (n = 8) and the Diabetic (n = 17). Diabetes mellitus was induced by intraperitoneal injection of streptozotocin. The diabetic animals were then randomly divided into two subgroups: diabetic rats without insulin treatment (n = 7) and diabetic rats with insulin treatment (n = 10). The neuronal nitric oxide synthase (nNOS) in the penile corpus cavernosum were assayed by immumohistochemical staining with specific antibody to nNOS and the nNOS-positive nerve fibers were counted semiquantitatively under a high power microscope.

Results:

The nNOS- positive nerve fibres in diabetic rats with treatment was higher than that in diabetic rats without treatment (P < 0.05) and lower than that in the controls (P < 0. 01). The nNOS-positive nerve fibres in diabetic rat without treatment were also lower than that in the controls (P < 0. 01).

Conclusion:

In streptozotocin-induced diabetic rats, the nNOS content in the penile corpus cavernosum was significantly decreased. Insulin treatment at the dose level employed partially restores the penile nNOS content in these rats.

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