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Related Experiment Videos

Characterization of coagulase from Staphylococcus intermedius.

Y Komori1, N Iimura, R Yamashita

  • 1Department of Microbiology, Faculty of Pharmacy, Meijo University, Nagoya, Japan.

Journal of Natural Toxins
|June 19, 2001
PubMed
Summary

Researchers isolated coagulase from Staphylococcus intermedius, a novel finding. This purified protein exhibits specific fibrinogen activity and stability, with tryptophan involvement suggested.

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Area of Science:

  • Microbiology
  • Protein Biochemistry

Background:

  • Staphylococcus species are known for producing coagulase, an enzyme with significant diagnostic and pathogenic relevance.
  • Coagulase activity is a key characteristic used in the identification and differentiation of Staphylococcus species.
  • Limited information exists regarding coagulase from Staphylococcus intermedius.

Purpose of the Study:

  • To isolate and characterize coagulase from Staphylococcus intermedius.
  • To determine the biochemical properties and substrate specificity of the purified coagulase.
  • To provide the first detailed report of coagulase from this specific bacterial species.

Main Methods:

  • Protein purification using affinity chromatography (bovine prothrombin-Sepharose 4B) and size exclusion chromatography (Bio-gel P-4).

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  • Homogeneity assessment via polyacrylamide gel electrophoresis (PAGE) and isoelectric focusing (IEF).
  • Characterization of biochemical properties including molecular weight, isoelectric point, amino acid composition, and stability.

    • Main Results:

    • A homogeneous coagulase protein was successfully isolated from Staphylococcus intermedius.
    • The purified coagulase has a molecular weight of 64,500 Da, an isoelectric point of 4.1, and 615 amino acid residues.
    • The enzyme demonstrated activity against human and rabbit fibrinogen but not rat or guinea pig fibrinogen, indicating species-specific substrate interaction. Tryptophan involvement in activity was suggested by NBS inhibition.

    Conclusions:

    • This study reports the first successful isolation and characterization of coagulase from Staphylococcus intermedius.
    • The purified coagulase exhibits distinct biochemical properties and specific fibrinogen activity.
    • The findings contribute to a better understanding of Staphylococcus virulence factors and bacterial enzyme diversity.