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Published on: March 16, 2017
Cloning, cellular distribution and functional expression of small intestinal epithelium guinea pig ClC-5 chloride
I Cornejo1, M I Niemeyer, F V Sepúlveda
1Centro de Estudios Cientificos, Casilla, Valdivia, Chile.
Abstract:
We report the cloning of a guinea pig ClC-5 chloride channel (gpClC-5) from distal small intestinal epithelial cells by RT-PCR and RACE. The transcript is shown to be present in duodenum, jejunum and ileum epithelium by RT-PCR and Northern analysis. This is confirmed by in situ hybridisation which also shows the transcript to be homogeneously distributed in the crypt and villus regions. Expression of gpClC-5 in HEK-293 cells generated markedly outwardly rectified chloride currents with a perm-selectivity sequence of NO(3)(-)>Cl(-)>Br(-)>I(-)>F(-)>gluconate(-). The possible role of gpClC-5 in this epithelial location is discussed.

