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Cloning and expression pattern of the human NDRG3 gene
1State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Sciences, Fudan University, Shanghai 200433, People's Republic of China.
Biochimica Et Biophysica Acta
|June 19, 2001
Summary
Researchers cloned and characterized N-myc downstream-regulated gene 3 (NDRG3). This gene is highly expressed in reproductive organs and may play a role in spermatogenesis.
Area of Science:
- Genetics and Molecular Biology
- Reproductive Biology
Background:
- The N-myc downstream-regulated gene (NDRG) family has members involved in cellular processes.
- Understanding novel NDRG family members can elucidate new biological pathways.
Purpose of the Study:
- To clone and characterize a novel gene, NDRG3.
- To investigate the expression pattern and potential function of NDRG3.
Main Methods:
- Gene cloning and sequencing of NDRG3.
- Northern blot analysis for gene expression.
- In situ hybridization for mRNA localization.
Main Results:
- NDRG3 cDNA is 2588 bp, encoding a 363 amino acid protein.
- NDRG3 is highly expressed in testis, prostate, and ovary.
- NDRG3 mRNA is localized to seminiferous epithelium.
Conclusions:
- NDRG3 is a novel gene with significant expression in reproductive tissues.
- NDRG3 may play a crucial role in the process of spermatogenesis.