Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

New views of BCR structure and organization.

L Matsuuchi1, M R Gold

  • 1Department of Zoology, University of British Columbia, 6270 University Boulevard, Vancouver, V6T 1Z4, British Columbia, Canada. matsuchi@zoology.ubc.ca

Current Opinion in Immunology
|June 19, 2001
PubMed
Summary

The B cell receptor (BCR) may exist as pre-assembled signaling complexes on resting cells and form larger arrays after antigen binding. BCR organization is crucial for B cell function and is altered in B cell tumors.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Noninvasive tissue adhesive for cardiac implantable electronic device pocket closure: the TAPE pilot study.

Journal of interventional cardiac electrophysiology : an international journal of arrhythmias and pacing·2018
Same author

The Rap GTPases regulate the migration, invasiveness and in vivo dissemination of B-cell lymphomas.

Oncogene·2009
Same author

Predicting geographical variations in behavioural risk factors: an analysis of physical and mental healthy days.

Journal of epidemiology and community health·2004
Same author

T wave alternans for ventricular arrhythmia risk stratification.

Minerva cardioangiologica·2003
Same author

Activation and function of the Rap1 GTPase in B lymphocytes.

International reviews of immunology·2002
Same author

Clinical experience with a dual-chamber implantable cardioverter defibrillator to treat atrial tachyarrhythmias.

Journal of cardiovascular electrophysiology·2002

Area of Science:

  • Immunology
  • Cell Biology
  • Molecular Biology

Background:

  • The B cell receptor (BCR) controls B cell activation and function.
  • Understanding BCR organization and signaling is key to B cell immunology.

Purpose of the Study:

  • To elucidate the stoichiometry and organization of the BCR.
  • To investigate BCR structure before and after antigen engagement.
  • To explore the role of BCR organization in B cell tumors.

Main Methods:

  • Stoichiometric analysis of BCR subunits.
  • Microscopy techniques to visualize BCR organization.
  • Biochemical assays to study signaling complex formation.

Main Results:

Related Experiment Videos

  • BCR may exist as pre-assembled oligomeric complexes on resting cells, generating basal signaling.
  • Antigen binding induces BCR organization into larger arrays within lipid rafts.
  • Signaling enzymes are concentrated at these BCR-associated lipid raft sites.
  • Altered BCR assembly and organization are observed in B cell tumors.

    • Conclusions:

    • BCR stoichiometry and organization are dynamic processes critical for B cell signaling.
    • Lipid rafts play a significant role in concentrating signaling molecules upon BCR activation.
    • Dysregulation of BCR organization is implicated in B cell malignancies.