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Related Experiment Videos

Nuclear-receptor interactions on DNA-response elements.

S Khorasanizadeh1, F Rastinejad

  • 1Department of Biochemistry and Molecular Genetics, School of Medicine, University of Virginia, Charlottesville, VA 22908-0733, USA.

Trends in Biochemical Sciences
|June 19, 2001
PubMed
Summary

Nuclear receptors bind DNA response elements to control gene transcription. Structural studies reveal how these receptors recognize DNA sequences and cooperate for enhanced binding selectivity.

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Area of Science:

  • Molecular biology
  • Structural biology
  • Genetics

Background:

  • Nuclear receptors are crucial transcription factors that regulate gene expression.
  • They bind to specific DNA sequences known as response elements.
  • These elements often feature conserved hexameric sequences in various arrangements.

Purpose of the Study:

  • To elucidate the structural mechanisms by which nuclear receptors recognize DNA.
  • To understand how receptor-DNA interactions are influenced by the configuration of response elements.
  • To investigate the structural basis for cooperative binding between nuclear receptors.

Main Methods:

  • X-ray crystallography and other structural biology techniques were employed.
  • Analysis of nuclear receptor-DNA binding complexes.

Related Experiment Videos

  • Comparative structural analysis of different DNA response element configurations.

    • Main Results:

    • Structural studies illustrate receptor strategies for recognizing DNA binding site symmetry and sequence.
    • Receptor-DNA complexes reveal how specific hexameric sequences are identified.
    • Cooperative binding mechanisms that enhance affinity and selectivity were identified.

    Conclusions:

    • Nuclear receptors employ specific structural strategies to bind DNA response elements.
    • The configuration of DNA elements influences receptor recognition and binding.
    • Cooperation between receptors is structurally underpinned, improving target site recognition.