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Antimicrobial study of bark from five tree species
S Pérez G1, M A Zavala S, L Arias G
1Universidad Autónoma Metropolitana-Xochimilco Depto. Sistemas Biológicos A.P. 23-181 México D.F.
Phytotherapy Research : PTR
|June 15, 2001
Summary
Methanol extracts from Lysiloma species and Gliricidia sepium demonstrated antimicrobial activity against several bacteria. Lysiloma tergemina showed significant bacteriostatic effects, highlighting its potential in antimicrobial research.
Area of Science:
- Pharmacognosy
- Microbiology
Background:
- Plant-derived compounds are a rich source of antimicrobial agents.
- Several plant species, including Lysiloma and Gliricidia, are traditionally used for medicinal purposes.
Purpose of the Study:
- To evaluate the antimicrobial potential of various plant extracts against a panel of pathogenic microorganisms.
- To identify specific plant species and extracts exhibiting significant inhibitory activity.
Main Methods:
- Extraction of plant bark using chloroform, methanol, and aqueous solvents.
- In vitro antimicrobial susceptibility testing against 12 different microbial strains (bacteria and fungi).
- Determination of minimum inhibitory concentrations (MICs) for active extracts.
Main Results:
- Methanol extracts from Lysiloma divaricata, Lysiloma tergemina, and Gliricidia sepium showed antimicrobial activity against Staphylococcus epidermidis, Staphylococcus aureus, Pseudomonas aeruginosa, Bacillus pumilus, and Vibrio cholerae at 200 µg/mL.
- Lysiloma tergemina exhibited the most potent effect, demonstrating a bacteriostatic impact on Staphylococcus epidermidis at 400 µg/mL.
Conclusions:
- Methanol extracts of Lysiloma species and Gliricidia sepium possess notable antimicrobial properties.
- Lysiloma tergemina is a promising candidate for further investigation as a natural antimicrobial agent.