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Myosin vb is associated with plasma membrane recycling systems
L A Lapierre1, R Kumar, C M Hales
1Departments of Medicine, Surgery, and Cellular Biology and Anatomy, Institute of Molecular Medicine and Genetics, Medical College of Georgia and the Augusta VA Medical Center, Augusta, Georgia 30912, USA.
Molecular Biology of the Cell
|June 16, 2001
Summary
Myosin Vb interacts with Rab11a, a key protein in the plasma membrane recycling system. This interaction is crucial for vesicle transport, as demonstrated by experiments showing myosin Vb
Area of Science:
- Cell biology
- Molecular biology
- Biochemistry
Background:
- Myosin Va is linked to vesicle populations, but myosin Vb's function remains unclear.
- Rab11a is a marker for plasma membrane recycling systems.
Purpose of the Study:
- To investigate the function of myosin Vb in cellular recycling pathways.
- To determine the interaction between myosin Vb and the Rab11 family of proteins.
Main Methods:
- Yeast two-hybrid screening to identify interacting proteins.
- Co-localization studies using GFP-tagged proteins and immunofluorescence.
- Overexpression of myosin Vb tail chimera to assess dominant-negative effects on vesicle trafficking.
Main Results:
- Myosin Vb interacts with Rab11a, Rab11b, and Rab25.
- Myosin Vb co-localizes with Rab11a in HeLa and MDCK cells, and its distribution is sensitive to microtubule-disrupting agents.
- Overexpression of the myosin Vb tail disrupts transferrin recycling and polymeric IgA receptor trafficking, causing vesicle accumulation.
Conclusions:
- Myosin Vb is associated with the plasma membrane recycling system in both nonpolarized and polarized cells.
- Myosin Vb plays a critical role in the transit of vesicles out of recycling compartments.