Related Experiment Video

Updated: Aug 15, 2026

Production of Pseudotyped Particles to Study Highly Pathogenic Coronaviruses in a Biosafety Level 2 Setting
08:40

Production of Pseudotyped Particles to Study Highly Pathogenic Coronaviruses in a Biosafety Level 2 Setting

Published on: March 1, 2019

Murine sarcoma virus related nucleic acid sequences in a non-transforming virus derived from an interspecies

H Okabe, R V Gilden, M Hatanaka

    International Journal of Cancer
    |May 15, 1975
    PubMed

    Insights

    This study identifies Moloney sarcoma virus sequences within a non-transforming virus (GLOH-minus), suggesting transformation ability loss due to sequence changes, not origin. This impacts understanding sarcoma virus evolution.

    Area of Science:

    • Virology
    • Molecular Biology
    • Oncology

    Background:

    • Sarcomagenic viruses can exhibit interspecies pseudotype characteristics.
    • Understanding viral sequence origins is crucial for oncogenic virus research.

    Purpose of the Study:

    • To determine the origin of mouse-specific sequences in the non-transforming GLOH-minus virus.
    • To elucidate the role of specific viral sequences in sarcomagenic transformation.

    Main Methods:

    • Molecular hybridization techniques were used to analyze viral nucleic acid sequences.
    • Thermal stability of inter-strain hybrid molecules differentiated between Gross and Moloney viruses.
    • Interspecies pseudotype analysis in rat-mouse systems.

    Main Results:

    • The non-transforming GLOH-minus virus retains mouse-specific sequences.
    • These sequences were identified as Moloney sarcoma virus-derived, not Gross virus-derived.
    • GLOH-minus, despite lacking transforming ability, shows high relatedness to Moloney sarcoma virus.

    Conclusions:

    • The mouse virus-specific sequences in GLOH-minus originate from Moloney sarcoma virus.
    • The loss of transforming ability in GLOH-minus is likely due to alterations or loss of critical transformation-related sequences.
    • This finding provides insight into the mechanisms of viral oncogenesis and evolution.

    More Related Videos

    In Vitro Establishment of a Genetically Engineered Murine Head and Neck Cancer Cell Line using an Adeno-Associated Virus-Cas9 System05:45

    In Vitro Establishment of a Genetically Engineered Murine Head and Neck Cancer Cell Line using an Adeno-Associated Virus-Cas9 System

    Published on: January 9, 2020

    Procedures for the Identification of SARS-CoV-2 Entry Inhibitors as Potential Antivirals using MLV-Based Pseudoviruses11:43

    Procedures for the Identification of SARS-CoV-2 Entry Inhibitors as Potential Antivirals using MLV-Based Pseudoviruses

    Published on: February 27, 2026

    Related Concept Videos

    Viral Mutations00:36

    Viral Mutations

    A mutation is a change in the sequence of bases of DNA or RNA in a genome. Some mutations occur during replication of the genome due to errors made by the polymerase enzymes that replicate DNA or RNA. Unlike DNA polymerase, RNA polymerase is prone to errors because it is not capable of “proofreading” its work. Viruses with RNA-based genomes, like HIV, therefore accrue mutations faster than viruses with DNA-based genomes. Because mutation and recombination provide the raw material for adaptive...
    Mechanisms of Retrovirus-induced Cancers01:51

    Mechanisms of Retrovirus-induced Cancers

    Retroviruses are RNA viruses that have been shown to cause cancers in diverse species, including chickens, mice, cats, and monkeys. The RNA genomes of these viruses are first reverse-transcribed into single and then double-stranded DNA (dsDNA) copies. This dsDNA called proviral DNA then integrates into the host genome. Subsequently, the host cell transcribes the proviral DNA in concert with the chromosomal DNA. This leads to the production of viral RNA and proteins that assemble at the host...
    Rous Sarcoma Virus (RSV) and Cancer01:03

    Rous Sarcoma Virus (RSV) and Cancer

    Rous Sarcoma virus or RSV was discovered by F. Peyton Rous in the year 1911 as a filterable transmissible agent that could cause tumors in chickens. He won a Nobel Prize for this discovery in 1966. His experiments clearly demonstrated that some cancers could be caused by infectious agents and led to the discovery of many more cancer-causing viruses in animals as well as humans.
    RSV is a retrovirus that contains two copies of a plus-strand  RNA genome. Its genome consists of four main open...
    Rous Sarcoma Virus (RSV) and Cancer01:03

    Rous Sarcoma Virus (RSV) and Cancer

    Rous Sarcoma virus or RSV was discovered by F. Peyton Rous in the year 1911 as a filterable transmissible agent that could cause tumors in chickens. He won a Nobel Prize for this discovery in 1966. His experiments clearly demonstrated that some cancers could be caused by infectious agents and led to the discovery of many more cancer-causing viruses in animals as well as humans.
    RSV is a retrovirus that contains two copies of a plus-strand  RNA genome. Its genome consists of four main open...
    Subviral Agents01:29

    Subviral Agents

    Subviral agents are infectious entities that resemble viruses but lack one or more viral components, such as a capsid or essential replication machinery. These agents include viroids, prions, and satellites, each possessing distinct structural and functional characteristics that influence their mode of infection and replication.Viroids are the simplest subviral agents, consisting of circular, single-stranded RNA molecules without a protein coat. They exclusively infect plants, relying entirely...
    Rabies01:28

    Rabies

    Rabies is a lethal zoonotic disease caused by a single-stranded, negative-sense RNA virus of the Lyssavirus genus, within the family Rhabdoviridae. Its primary mode of transmission to humans is through bites or saliva-contaminated scratches from infected mammals such as dogs, bats, raccoons, or foxes. Transmission can also occur if infectious saliva contacts abraded skin or intact mucous membranes, including the conjunctiva.Viral Entry and Early ReplicationOnce introduced at the bite or scratch...