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Basic fibroblast growth factor up-regulates the surface expression of complement receptors on human monocytes
1Department of Transfusion Medicine, Juntendo University School of Medicine, Tokyo, Japan. ohsaka@med.juntendo.ac.jp
Summary
Basic fibroblast growth factor (b-FGF) enhances complement receptor (CR) expression on human monocytes, suggesting a role in inflammation. This study investigated b-FGF
Area of Science:
- Immunology
- Cell Biology
- Inflammation Research
Background:
- Basic fibroblast growth factor (b-FGF) is implicated in various biological processes.
- Complement receptors (CR) on monocytes play a crucial role in inflammatory responses.
Purpose of the Study:
- To investigate the effect of b-FGF on complement receptor expression on human monocytes.
- To elucidate the potential involvement of b-FGF in inflammatory mechanisms.
Main Methods:
- Human monocytes were isolated from healthy donors.
- Surface expression of CR1 and CR3 was analyzed using flow cytometry and immunofluorescence.
- A whole blood lysis technique was employed to maintain monocyte integrity.
Main Results:
- b-FGF significantly upregulated the expression of CR3 and CR1 on monocytes in a dose- and time-dependent manner.
- Maximal stimulation was observed with b-FGF concentrations >25 ng/ml and incubation periods of 90-120 minutes.
- CR3 expression showed a greater increase compared to CR1 expression.
Conclusions:
- b-FGF modulates complement receptor expression on human monocytes.
- These findings suggest that b-FGF may play a role in inflammatory processes by influencing monocyte CR expression.