Changes in BiP (GRP78) levels upon HSV-1 infection are strain dependent
H Mao1, D Palmer, K S Rosenthal
1Northeastern Ohio Universities College of Medicine, 4209 State Route 44, Box 95, Rootstown, OH 44272, USA.
Virus Research
|June 19, 2001
Summary
Different herpes simplex virus type 1 (HSV-1) strains affect the chaperone protein BiP (grp78) levels differently. Neuroinvasive HSV-1 strains increase BiP, while attenuated strains decrease it, impacting viral-host interactions.
Area of Science:
- Cellular biology
- Virology
- Molecular biology
Background:
- BiP (grp78) is a crucial chaperone protein regulating the unfolded protein response.
- HSV-1 infection involves complex interactions with host cell machinery.
Purpose of the Study:
- To investigate the impact of different HSV-1 strains on BiP protein levels and synthesis.
- To understand how viral strain variations influence BiP concentration during infection.
Main Methods:
- Comparing BiP levels in cells infected with neuroinvasive (SP7, 490) versus attenuated (KOS) HSV-1 strains.
- Analyzing BiP protein synthesis, sequestration, and degradation patterns.
Main Results:
- BiP levels increased in cells infected with neuroinvasive HSV-1 strains (SP7, 490).
- BiP levels decreased in cells infected with the attenuated KOS strain.
- SP7 infection showed sustained BiP synthesis and limited degradation, while KOS infection led to decreased synthesis and degradation.
Conclusions:
- HSV-1 strain-dependent differences in BiP concentration significantly affect viral-host cell interactions.
- Modulation of BiP by HSV-1 may play a role in viral neuroinvasiveness and pathogenesis.
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