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Related Experiment Videos

The lac operator-repressor system is functional in the mouse.

C A Cronin1, W Gluba, H Scrable

  • 1Department of Neuroscience, University of Virginia, Charlottesville, VA 22908, USA.

Genes & Development
|June 19, 2001
PubMed
Summary
This summary is machine-generated.

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Scientists successfully transferred the lac operator-repressor system to mice, enabling reversible control of gene expression. This breakthrough allows for precise regulation of mammalian genes, including pigmentation, using the lac repressor and IPTG.

Area of Science:

  • Molecular Biology
  • Genetics
  • Mammalian Gene Regulation

Background:

  • The lac operator-repressor system is a well-established gene regulation tool in bacteria.
  • Mammalian gene regulation offers complex control mechanisms but lacks simple, reversible external triggers.
  • Integrating prokaryotic regulatory systems into mammalian genomes presents significant challenges.

Purpose of the Study:

  • To establish a functional bacterial gene regulatory system (lac operator-repressor) in a mammalian model (mouse).
  • To demonstrate reversible control over a mammalian reporter gene using this integrated system.
  • To explore the potential for precise, externally controlled gene expression in mammals.

Main Methods:

  • Construction of a lac repressor transgene optimized for mammalian expression (codon usage, structure).

Related Experiment Videos

  • Development of a mammalian reporter gene (tyrosinase) containing lac operator sequences within its promoter.
  • Transduction of the repressor transgene into mice and assessment of reporter gene activity and reversibility.

    • Main Results:

    • The lac repressor transgene was successfully expressed at functional levels in mice.
    • Expression of the tyrosinase reporter gene was effectively regulated by the lac repressor.
    • Gene expression and resulting mouse pigmentation were reversibly controlled by the lactose analog IPTG.

    Conclusions:

    • A functional bacterial lac operator-repressor gene regulatory system can be transferred and operate effectively in mice.
    • This system enables tight, reversible control over mammalian gene expression, demonstrated by pigmentation changes.
    • The findings hold promise for developing novel methods for controlling endogenous mammalian gene expression.