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Radioactive in situ Hybridization for Detecting Diverse Gene Expression Patterns in Tissue
17:38

Radioactive in situ Hybridization for Detecting Diverse Gene Expression Patterns in Tissue

Published on: April 27, 2012

Protease XXIV increases detection of mucin gene expression during in situ hybridization in archival tissue

J M Rheinhardt1, W E Finkbeiner

  • 1Department of Pathology, University of California-Davis, Davis, California, USA. jmrheinhardt@ucdavis.edu

The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society
|June 19, 2001
PubMed

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In-situ Hybridization02:31

In-situ Hybridization

In situ hybridization (ISH) is a technique used to detect and localize specific DNA or RNA molecules in cells, tissue, or tissue sections using a labeled probe. The technique was first used in 1969 for the investigation of nucleic acids. It is currently an essential tool in scientific research and clinical settings, especially for diagnostic purposes.
Types of probes and labels
A probe is a complementary strand of DNA or RNA that binds to corresponding nucleotide sequences in a cell. Many...