[Study on fermentation of long-chain dicarboxylic acid based on enzymology]

In this paper, we studied the effects of the adding time of alkane on the expression of P450 and production of dicarboxylic acid. A novel fermentation process, in which no or a little alkane was added to make the cells growing more quickly during the growth stage, followed by the addition of alkane to induce cytochromes P450 for 6-8 hours, was established. The results showed that the new process was much better than the old process on inductivities of cytochromes P450 and production of dicarboxylic acid. The new process improved nearly 14.56% expression of P450 and 14.15% production of dicarboxylic acid.