A screening method for sulfonamides extracted from animal tissues.

Area of Science:

• Analytical Chemistry
• Food Science
• Veterinary Medicine

Background:

• Sulfonamides are commonly used antibiotics in poultry farming.
• Ensuring the absence of sulfonamide residues in poultry products is crucial for public health and regulatory compliance.
• Existing methods for residue detection may lack sensitivity or be prone to interference.

Purpose of the Study:

• To develop and validate a sensitive screening method for estimating sulfonamide residues in poultry tissues.
• To provide a reliable analytical approach for quality control in the poultry industry.
• To differentiate between simple and complex matrices for accurate sulfonamide quantification.

Main Methods:

• Utilized thin layer chromatography (TLC) for the separation of Bratton-Marshall positive reactants.
• Employed a colorimetric method for quantitation in the absence of interfering substances.
• Applied TLC for both qualitative and quantitative analysis when interferences were present.

Main Results:

• The developed screening method demonstrates high sensitivity, detecting residues below 0.05 ppm.
• Achieved a recovery rate greater than 80% for sulfonamide residues.
• Successfully differentiated analytical approaches based on the presence or absence of interfering compounds.

Conclusions:

• The described method provides a reliable and sensitive approach for screening sulfonamide residues in poultry.
• The combined use of TLC and colorimetry offers flexibility for analyzing diverse poultry tissue matrices.
• This method contributes to enhanced food safety and regulatory monitoring of poultry products.