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A screening method for sulfonamides extracted from animal tissues.

W F Phillips, J E Trafton

    Journal - Association of Official Analytical Chemists
    |January 1, 1975
    PubMed
    Summary

    This study presents a new screening method for detecting sulfonamide residues in poultry. The method uses thin layer chromatography (TLC) and colorimetry, offering high sensitivity for ensuring food safety.

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    Area of Science:

    • Analytical Chemistry
    • Food Science
    • Veterinary Medicine

    Background:

    • Sulfonamides are commonly used antibiotics in poultry farming.
    • Ensuring the absence of sulfonamide residues in poultry products is crucial for public health and regulatory compliance.
    • Existing methods for residue detection may lack sensitivity or be prone to interference.

    Purpose of the Study:

    • To develop and validate a sensitive screening method for estimating sulfonamide residues in poultry tissues.
    • To provide a reliable analytical approach for quality control in the poultry industry.
    • To differentiate between simple and complex matrices for accurate sulfonamide quantification.

    Main Methods:

    • Utilized thin layer chromatography (TLC) for the separation of Bratton-Marshall positive reactants.
    • Employed a colorimetric method for quantitation in the absence of interfering substances.
    • Applied TLC for both qualitative and quantitative analysis when interferences were present.

    Main Results:

    • The developed screening method demonstrates high sensitivity, detecting residues below 0.05 ppm.
    • Achieved a recovery rate greater than 80% for sulfonamide residues.
    • Successfully differentiated analytical approaches based on the presence or absence of interfering compounds.

    Conclusions:

    • The described method provides a reliable and sensitive approach for screening sulfonamide residues in poultry.
    • The combined use of TLC and colorimetry offers flexibility for analyzing diverse poultry tissue matrices.
    • This method contributes to enhanced food safety and regulatory monitoring of poultry products.

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