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Collaborative study of a spectrofluorometric method for lasalocid sodium in feeds
Journal - Association of Official Analytical Chemists
|May 1, 1975
Summary
A new spectrofluorometric method accurately determines lasalocid sodium in poultry feed. This validated method is rapid, sensitive, and free of interference, ensuring reliable feed additive analysis.
Area of Science:
- Analytical Chemistry
- Food Science
- Veterinary Pharmacology
Background:
- Accurate quantification of feed additives like lasalocid sodium is crucial for animal health and regulatory compliance.
- Existing methods for lasalocid sodium determination may lack sensitivity or be prone to interference.
Purpose of the Study:
- To develop and validate a rapid, sensitive, and interference-free spectrofluorometric method for quantifying lasalocid sodium in finished poultry feeds.
- To establish a collaborative study to assess the method's reliability across multiple laboratories.
Main Methods:
- Samples were treated with pH 4.7 buffer and extracted using ethyl acetate.
- Extracts underwent cleanup with hydrochloric acid (HCl) and sodium hydroxide (NaOH).
- Quantitative measurement was performed via spectrofluorometry, with nonspecific fluorescence corrected using a lasalocid-boric acid complex.
Main Results:
- The method demonstrated high accuracy, with average recoveries of lasalocid sodium at 100%, 98%, and 99% for different addition levels.
- Coefficients of variation were low, ranging from 8.0% to 12.8%, indicating good precision.
- The method showed no interference from common feed additives such as monensin and ethoxyquin.
Conclusions:
- The developed spectrofluorometric method is reliable for determining lasalocid sodium in poultry feed.
- The method's adoption as official first action underscores its efficacy and suitability for routine analysis.
- This validated method contributes to improved quality control in animal feed production.