Related Experiment Video
Updated: Jul 28, 2026

Isolation of Labile Multi-protein Complexes by in vivo Controlled Cellular Cross-Linking and Immuno-magnetic Affinity Chromatography
Published on: March 9, 2010
Separation of Listeria from cheese and enrichment media using antibody-coated microbeads and centrifugation
E Kaclíková1, T V Kuchta, H Kay
1Department of Microbiology and Chemistry, Food Research Institute, Priemyselná 4, PO Box 25, SK-82475 26, Bratislava, Slovak Republic.
Abstract:
An immunoseparation system for the separation of Listeria from enriched cheese samples was developed. The system utilizes polystyrene microbeads (3.8 microm in diameter) coated with covalently bound anti-Listeria genus-specific antibody. The beads were incubated with cheese enriched in half-Fraser broth and the bead-bacterial complex was separated by centrifugation at 110 g then spread on selective agar plates. Although cross-reactivity with certain Gram-positive bacteria (Staphylococcus saprophyticus and Arthrobacter sp.) was determined, this had no negative effect on capture effectiveness of the beads to Listeria spp. The minimum density of Listeria cells positively detected by immunoseparation with subsequent plating was 10(0) cfu/ml. The application of the separation method facilitates a reduction in the time of Listeria detection in cheese by 2 days without affecting the sensitivity.
More Related Videos
13:49Semi-automated Biopanning of Bacterial Display Libraries for Peptide Affinity Reagent Discovery and Analysis of Resulting Isolates
Published on: December 6, 2017
06:39Isolation and Purification of Bacterial Extracellular Vesicles from Human Feces Using Density Gradient Centrifugation
Published on: September 1, 2023
Related Concept Videos
Overview Of Cell Separation And Isolation
Centrifugation