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Updated: Aug 4, 2026

'Bioluminescent' Reporter Phage for the Detection of Category A Bacterial Pathogens
Published on: July 8, 2011
Combination of ATP-bioluminescence and PNA probes allows rapid total counts and identification of specific
H Stender1, A Sage, K Oliveira
1Boston Probes, Inc., 75E Wiggins Avenue, 01730, Bedford, MA, USA. HStender@BostonProbes.com
Abstract:
We have combined ATP-dependent bioluminescence with a novel chemiluminescent in situ hybridization (CISH) method using peroxidase-labeled peptide nucleic acid (PNA) probes targeting species-specific rRNA sequences to provide total counts and subsequent identification of specific microorganisms. Both methods are applied to the same membrane filter following a short incubation time and both methods provide results in the form of spots of light that are captured by the MicroStar detection system. Each spot of light represents individual micro-colonies detected by either ATP bioluminescence or PNA CISH. This new concept is particularly intended for in process and quality control of non-sterile products to rapidly provide total counts as well as presence/absence of specific indicators and/or pathogens in non-sterile, filterable samples.
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