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A Protocol for Phage Display and Affinity Selection Using Recombinant Protein Baits
Published on: February 16, 2014
Methodology for optimizing functional miniature proteins based on avian pancreatic polypeptide using phage display
J W Chin1, R M Grotzfeld, M A Fabian
1Department of Chemistry, Yale University, New Haven, CT 06520-8107, USA.
Bioorganic & Medicinal Chemistry Letters
|June 20, 2001
Abstract:
Synthetic genes for avian pancreatic polypeptide (aPP) and for the miniature DNA binding protein PPBR4 were cloned and expressed on the surface of M13 bacteriophage. We anticipate that these constructs will have utility optimizing the properties of miniature proteins based on aPP that result from our previously described protein grafting procedure.
