Related Experiment Videos
N-Me-pAB-Glu-gamma-Glu-gamma-Tyr(3-NO(2)): an internally quenched fluorogenic gamma-glutamyl hydrolase substrate
1Departments of Chemistry and Medicinal Chemistry, University of Michigan, Ann Arbor, MI 48109-1055, USA.
Bioorganic & Medicinal Chemistry Letters
|June 20, 2001
Abstract:
A gamma-glutamyl tripeptide containing an internally quenched fluorophore has been synthesized and shown to be a substrate for recombinant rat gamma-glutamyl hydrolase. HPLC, LC-MS, and fluorescence spectra support the conclusion that selective hydrolysis occurs at the penultimate peptide bond. Preliminary data indicate that hydrolysis of this substrate can be monitored continuously to yield steady-state kinetic data.