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Updated: Aug 10, 2026

In Vitro Assay to Measure Phosphatidylethanolamine Methyltransferase Activity
Published on: January 5, 2016
Phenylethanolamine N-methyltransferase kinetics: bovine versus recombinant human enzyme
G L Grunewald1, M J McLeish, K R Criscione
1Department of Medicinal Chemistry, University of Kansas, 4060 Malott Hall, 1251 Wescoe Hall Drive, Lawrence, KS 66045-7582, USA. ggrunewald@ukans.edu
Abstract:
Epinephrine (Epi) acts as a neurotransmitter in the brain, but its function therein is not well understood. Phenylethanolamine N-methyltransferase (PNMT) catalyzes the final step in the biosynthesis of Epi and is thus a pharmacological target to investigate the function of Epi in the central nervous system. The kinetic differences between bovine adrenal PNMT and human brain PNMT for a number of substrates and inhibitors are examined and the results reported.

