Related Experiment Video
Updated: Feb 17, 2026

Cortisol Extraction from Sturgeon Fin and Jawbone Matrices
Published on: September 10, 2019
Quantitative determination of cortisol in human plasma by high-pressure liquid chromatography
Abstract:
A method is described for the determination of cortisol in human plasma by high-pressure liquid chromatography. The simplified extraction procedure makes the method applicable to routine clinical assays. Partition chromatography is carried out on a Zorbax-Sil column with the eluent system dichloromethane-ethanol-water. A 78% recovery was obtained for cortisol. The detection limit is 1 mug per 100 ml in 1 ml of plasma. Cortisol values were determined in samples from a random selection of patients.
Related Concept Videos
High-Performance Liquid Chromatography: Introduction
In HPLC, two phases play a critical role in the separation process:
High-Performance Liquid Chromatography: Instrumentation
High-Performance Liquid Chromatography: Elution Process
Types Of Column Chromatography
Gel Filtration Chromatography
When the...
High-Performance Liquid Chromatography: Types of Detectors

