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Related Experiment Videos

Hemoglobin enhances tissue factor expression on human malignant cells.

F A Siddiqui1, A Amirkhosravi, M Amaya

  • 1Clinical and Research Laboratories, Walt Disney Memorial Cancer Institute, Florida Hospital, Orlando 32804, USA. Farooq_Siddiqui_Ph.D.@mail.fhmis.net

Blood Coagulation & Fibrinolysis : an International Journal in Haemostasis and Thrombosis
|June 21, 2001
PubMed
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Hemoglobin (Hb) binds to Tissue Factor (TF)-expressing cancer cells, increasing TF levels and blood clotting activity. This suggests Hb may promote metastasis through TF upregulation.

Area of Science:

  • Biochemistry
  • Oncology
  • Hematology

Background:

  • Tissue Factor (TF) initiates blood coagulation and is implicated in cancer metastasis.
  • Hemoglobin (Hb) has been shown to bind TF and enhance its procoagulant activity (PCA).

Purpose of the Study:

  • To investigate the effect of Hb on TF expression and PCA in human malignant cells.
  • To determine if Hb binding to TF leads to de novo synthesis of TF.

Main Methods:

  • Incubation of TF-expressing (A375, J82) and TF-negative (KG1) cells with varying Hb concentrations.
  • Analysis of Hb binding, TF expression, and PCA using flow cytometry, confocal microscopy, and immunoassay.
  • Assessment of TF synthesis inhibition using cycloheximide.

Main Results:

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  • Hb concentration-dependently bound to TF+ cells, increasing TF expression up to sixfold and enhancing PCA.
  • Increased TF expression and PCA were significantly inhibited by cycloheximide, indicating de novo synthesis.
  • Hb did not induce TF expression or PCA in TF- cells (KG1).

Conclusions:

  • Hb specifically binds to TF-bearing malignant cells, upregulating TF expression and PCA.
  • This upregulation appears to involve de novo synthesis of TF.
  • Further research is needed to elucidate the precise mechanisms of Hb-TF interaction and TF regulation.