Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Short tandem repeat profiling provides an international reference standard for human cell lines.

J R Masters1, J A Thomson, B Daly-Burns

  • 1Institute of Urology, University College London, 3rd Floor Research Laboratories, 67 Riding House Street, London W1W 7EY, United Kingdom. J.Masters@Ucl.ac.uk

Proceedings of the National Academy of Sciences of the United States of America
|June 21, 2001
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Effect of metabolic and antioxidant supplementation on sperm parameters in oligo-astheno-teratozoospermia, with and without varicocele: A double-blind placebo-controlled study.

Andrologia·2018
Same author

Preface.

International journal of radiation biology·2017
Same author

Histopathological and electrophysiological indices of rotenone-evoked dopaminergic toxicity: Neuroprotective effects of acetyl-L-carnitine.

Neuroscience letters·2015
Same author

Elevated expression of artemis in human fibroblast cells is associated with cellular radiosensitivity and increased apoptosis.

British journal of cancer·2012
Same author

Acetyl-L-Carnitine Modulates TP53 and IL10 Gene Expression Induced by 3-NPA Evoked Toxicity in PC12 Cells.

Current neuropharmacology·2011
Same author

Extended-term culture of human T lymphocytes: Material potentially useful in genotoxicity testing.

Toxicology in vitro : an international journal published in association with BIBRA·2010

Cell line cross-contamination leads to scientific misrepresentation. Short tandem repeat (STR) profiling offers a standardized, inexpensive DNA fingerprinting method to authenticate cell lines and prevent future errors.

Area of Science:

  • Biotechnology
  • Genetics
  • Cell Biology

Background:

  • Cell line cross-contamination is a significant issue in scientific research, leading to misrepresentation.
  • Up to 36% of cell lines may be misidentified, impacting research reproducibility and validity.
  • Standardized authentication methods are crucial for maintaining scientific integrity.

Purpose of the Study:

  • To evaluate the effectiveness of short tandem repeat (STR) profiling as a standard method for cell line authentication.
  • To assess the potential of STR profiling to mitigate scientific misrepresentation caused by cell line misidentification.
  • To promote international acceptance of STR profiling for cell line verification.

Main Methods:

  • Analysis of 253 human cell lines from global sources using short tandem repeat (STR) profiling.

Related Experiment Videos

  • STR profiling generates a unique numerical code for each cell line, serving as a reproducible identifier.
  • The method was tested for its cost-effectiveness and potential as an international standard.

    • Main Results:

    • Short tandem repeat (STR) profiling demonstrated its capability as a simple, reproducible, and inexpensive method for cell line authentication.
    • The numerical code generated by STR profiling can serve as an international reference standard for human cell lines.
    • Successful application of STR profiling across diverse cell lines from various institutions worldwide.

    Conclusions:

    • Widespread adoption and international demand for DNA profiling, specifically short tandem repeat (STR) profiling, can significantly reduce scientific misrepresentation due to cell line cross-contamination.
    • STR profiling provides a robust solution for ensuring cell line identity and origin.
    • Implementing STR profiling as a global standard will enhance the reliability and accuracy of biomedical research.